![]() Yen had a starring role in the film Iron Monkey in 1993. Yen's first step into the film industry was when he landed his first starring role in the 1984 film Drunken Tai Chi.Īfter filming Drunken Tai Chi and Tiger Cage (1988), Yen made his breakthrough role as General Nap-lan in Once Upon a Time in China II (1992), which included a fight scene between his character and Wong Fei-hung (played by Jet Li). From a young age, he was taught by his parents to play musical instruments, including the piano. His mother is a soprano, in addition to being a martial arts teacher in Boston, while his father is a violinist. Yen also came from a family of musicians. When Yen decided to return to the United States, he made a side-trip to Hong Kong, where he met action choreographer Yuen Woo-ping. At age 16, Yen began practising Wushu after his parents sent him to Beijing to train with the Beijing Wushu Team. His younger sister, Chris Yen, is also a martial artist and actress, and appeared in the 2007 film Adventures of Johnny Tao: Rock Around the Dragon.Īt a young age, under the influence of his mother, Yen developed an interest in martial arts and began experimenting with various styles, including t'ai chi and other traditional Chinese martial arts. When he was two years old, his family moved to Hong Kong and then to the United States, settling in Boston when he was 11. His mother, Bow-sim Mark, is a Fu Style Wudangquan (internal martial arts) and Tai Chi grandmaster, while his father, Klyster Yen (甄雲龍), was a newspaper editor. Yen was born on 27 July 1963 in Guangzhou, Guangdong, China. He has also gained international recognition for playing Chirrut Îmwe in Rogue One (2016) and Xiang in XXX: Return of Xander Cage (2017), as well as Commander Tung in Mulan (2020) and Caine in John Wick: Chapter 4 (2023). Ip Chun, the eldest son of Ip Man, even mentioned that he is grateful to Yen for making his family's art popular and allowing his father's legacy to be remembered. He portrays Wing Chun grandmaster Ip Man in the Ip Man film series, which has garnered box office success and led to an increase in the number of people taking up Wing Chun, with hundreds of new Wing Chun schools being opened up in mainland China and other parts of Asia. Yen is credited by many for contributing to the popularisation of Wing Chun in China. Yen earned HK$220 million (US$28.4 million) from four films and six advertisements in 2013. ![]() One of the most popular film stars in Asia of the early 2000s, Yen is consistently one of the highest-paid actors in Asia. ![]() Yen has displayed skill in an array of martial arts, being well-versed in Tai Chi, Boxing, Kickboxing, Jeet Kune Do, Hapkido, Mixed martial arts, Taekwondo, Karate, Muay Thai, Wrestling, Brazilian jiu-jitsu, Hung Ga, Sanda, Judo, Wing Chun, and Wushu. The first Chinese UFC champion Zhang Weili stated that Yen's films introduced her to MMA. Regarded as one of Hong Kong's top action stars, Yen is widely credited for bringing mixed martial arts (MMA) into mainstream Asian cinema by choreographing MMA in many of his films since the early 2000s. Donnie Yen Ji-dan ( Chinese: 甄子丹 born 27 July 1963) is a Hong Kong actor, martial artist, and action director.
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![]() We know that many live in apartments, condos, or homes that do not have screens and, because of that, they aren’t sure how to begin. We love teaching others how to approach each step from measuring windows to proper screen installation. If you need to install window screens, but aren’t sure how, see our how-to videos and guides. There are countless possibilities when you introduce window or solar screen to your home or business. Install blackout screen shades in your bedroom for undisturbed sleep. ![]() Protect windows from your pet’s claws with specially made pet guards and screens. Install entirely new screens or make repairs to existing ones with material rolls. No matter if you’re taking on home improvement projects alone or you own a window and screen shop, our wide-ranging inventory stocks even the smallest of parts like spring, clips, latches, spline rollers, frame notching jigs, and much more. You can truly find all of your window screen necessities here! From fully assembled screens to unassembled kits designed for DIYers, we cater to all doers and makers. For smaller windows, our retractable and adjustable window screen and screen doors are ideal. You can also find heat blocking solar screens in our inventory made of Textilene® mesh in 80% and 90% options. We also feature fiberglass and aluminum patio and porch screen panels. Our window screen replacement options include fiberglass, aluminum, No-See-Um, pet resistant, solar insect, and UltraVue. We offer an expansive selection of window and solar screens to improve the level of comfort both indoors and out. No matter what the dimensions are of your windows, you can easily buy window and solar screens for a perfect fit! With Quality Window Screen Co., you can find screens to cool your home, stand up to your pet’s claws, blackout entire rooms, and prevent insects from entering through your windows. ![]() Window And Solar Screen Replacements Supplier & Manufacturer ofĪs one of the top online suppliers and manufacturers of replacement screens for windows, we offer all of the right options you need to screen any space. ![]() One morning last week I was in a rush and didn’t want to use my fingers or concealer brush to apply concealer one option is messy, the other is time consuming. So when I saw a cheap oval brush, I finally picked one up for 10 RMB (that’s ~$1.70) at a store called “Nature Green” here in China. Thankfully, because they are so popular, they’re getting copied everywhere, quickly. You know those oval brushes that have been all over Instagram? Yeah they are hella expensive and nobody in their right mind would pay $150+ for a set of brushes. That’s how inventions are born anyway, right? That’s how I have been applying concealer for the past 3 years, until last week when I was too busy/lazy to do it right. It’s perfect.Īs for how to apply Stretch Concealer, the best way to apply any concealer is with a concealer brush, right? This concealer won’t smudge or budge, even with the additional abrasion of a foundation sponge / brush / whatever you use on top of it. I am here to tell you today that Glossier is switching up the game.Īpply your concealer first, then foundation on top. Now wait a second–most people will tell you to apply concealer after foundation to cover up blemishes, right? Yes, they do. My foundation–which is a powder compact–applies seamlessly over this concealer. With only 5 shades to choose from, the odds are not in your favor, but the shade “Light 10” was closer to my skin than any other concealers have been before.Ī concealer should be a shade lighter than your foundation color. In fact, I can safely say that Stretch Concealer is safe to wear on it’s own– that is, if it matches your skin tone. It lives up to its promise, by offering complete coverage without the 2-dimensional flatness that most concealers give your skin. Stretch Concealer does what others can’t: it is highly pigmented, glides on smoothly, and most importantly: doesn’t look flat or sink into fine lines. They definitely get the job done.īut getting the job done and getting it done right are not the same thing. Most concealers that I love– Benefit’s Booing, Stila’s Stay All Day Concealer–are very highly pigmented concealers. The first time I tried stretch concealer, I was immediately impressed. The product lives up to it’s description. In five shades painstakingly developed to enhance, brighten, and-most importantly-completely disappear into the widest range of skin tones.” The buildable formula covers everything from dark circles to redness and zits. Ours is a new type of concealer with elastic micro waxes that move with your face instead of caking on top of it, and nourishing oils that give a dewy, glowing finish. “A traditional concealer sets to a stiff, flat, dry finish-a dead giveaway that you’re wearing it. Glossier is literally the culmination of a decade of top beauty blogging, to the tactile end product.Īs for Stretch Concealer, it’s tagline is: “Makeup that lets skin look like…skin!” She was able to pick and choose what she liked, what worked, what didn’t. In other words: Glossier makes beauty a no-brainer.Īs the editor of Into the Gloss, one of the top beauty blogs in the game (if not the #1 beauty blog), Weiss had the opportunity to test and try every beauty product known to woman, with a preference for the high-end. From skincare to makeup, they’ve narrowed it down to the best possible products in the least number of options. That being said, this concealer should be your first choice. ![]() Their tagline? Skincare first, makeup second. ( Here’s a recent interview with her that I drooled over). Glossier is the newest 80s/9os retro chic minimalist beauty brand, founded by badass entrepreneur Emily Weiss of Into the Gloss. Today I want to introduce you to the end-all be-all for concealers: Glossier Stretch Concealer. If any questions arise related to the information contained in the translated website, please refer to the English version. Any discrepancies or differences created in the translation are not binding and have no legal effect for compliance or enforcement purposes. The web pages currently in English on the DMV website are the official and accurate source for the program information and services the DMV provides. ![]() (the permission of the exporter may or may not be. The DMV is unable to guarantee the accuracy of any translation provided by Google™ Translate and is therefore not liable for any inaccurate information or changes in the formatting of the pages resulting from the use of the translation application tool. This information can thus be obtained from the appropriate government agency. Google™ Translate is a free third-party service, which is not controlled by the DMV. This translation application tool is provided for purposes of information and convenience only. If you are involved in a traffic collision and no one is injured, you are required to complete and submit a written report (SR1) to. With all that together, and your developed knowledge of escape ramps, you’ll be all set.The Department of Motor Vehicles (DMV) website uses Google™ Translate to provide automatic translation of its web pages. On the side of the freeway in an emergency. Then you’ll also need to make sure you have a valid driver’s license. The CA written driver’s test includes questions about traffic signals, so be sure to test yourself beforehand with a DMV.com FREE practice exam. After every question on the practice test, you will get instant feedback with a brief explanation if your answer is incorrect. Not to mention, you will get a ticket with a hefty fine if caught running a red light. Each CA DMV practice test at has 25 random questions based on the handbook and authentic questions from California DMV ( dmv.ca.gov ). Your social security card or birth certificate will be sufficient. Traffic Signal Responding to traffic signals in the proper way is essential for safe driving. Then you also need to have proof of citizenship or permanent residency. Use your parking brakes and leave the vehicle in gear or 'park'. C.) Your front wheels should be parallel to the road if there is no curb. ![]() B.) One of your rear wheels should touch the curb. Certificates last two years unless you have a condition that needs monitoring, like high blood pressure. A.) Use your parking brakes and leave the vehicle in gear or 'park'. Are you medically fit to drive a commercial vehicle? You’ll have to prove it with a certificate from a licensed medical examiner, as listed on the Federal Motor Carrier Safety Administration (FMCSA) National Registry. If you want to avoid multiple trips, you also need to be ready with all the qualifying paperwork. turn on your emergency flashers and pull off the road. you suspect your automobile has encountered a mechanical problem. ![]() turn your front wheels toward the curb when you park. your tires will start to slide over the water. Get just 10 wrong and score below an 80% and you are looking at another trip to the DMV on another day. if you drive too fast on a wet road, there is a danger that. The exam can be tricky and you only have an hour to answer the 50 questions. How much do you know about escape ramps or how to assess the proper following distance behind another vehicle? Whatever you think you already know, you’ll want to check your knowledge against the official California Commercial Driver’s Manual and take some practice tests to hone your answers. If you are involved in a traffic collision and no one is injured, you are required to complete and submit a written report (SR1) to: EDD. ![]() Before heading to the California DMV to take the General Knowledge exam to receive your Commercial Driver’s License there are a few things you’ll want to consider. You may not park your vehicle: On the side of the freeway in an emergency. ![]() If you forgo lithium, then MotoBatt AGM batteries also have 4 terminals for the same setup. Then on the other set of cable connectors, connect your perfected brand of lithium battery charger - SAE pigtail - to keep the battery charged and fresh. This provides a direct shot of the lithium jumper to the bike starting system if needed.if the RS restart button is a no go. The bike battery cables and the EC5 female pigtail go together at the main set of battery connectors. The most bullet proof setup going IMO is Antigravity lithium with a Re Start button on top and terminal 4 connectors (extra set) design. First thing I usually do when I get a new vehicle is put a pigtail connector for a battery tender on the battery. Hope this helps and you've learned something about EC5 connectors?Ĭlick to expand.Alot of the solutions are how accessible the battery is and how often are you needing to jump it?Ī small dirtbike battery that is on it's way out or has been started to much (say a flooded bike) that it needs more battery to get going and get home is one thing.Ī battery on a bike for a long ride out on tour that needs a jump every time to get home or get down the road till another battery is sourced is another thing.Īn easily accessible female EC5 pigtail is always going to be the ideal solution. Use a non AG pigtail or bolt an additional SAE pigtail to your battery for charging. No idea what happens when the Antigravity pigtail with the extra 1 way box is used?Īs modern battery chargers need a 2 way power/test back and forth to charge, that AG pigtail might be a problem? You thus can use this to quickly plug in your battery charger box to your bike w/ that EC5 pigtail attached. This serves as a redundant battery backup to the lithium jumper.every bike a potential jumper. When a bike has a poor start they pull out the cable fast from a tank bag and quickly connect 2 bikes together. ![]() They can easily use a jumper fast as needed.but they also brings a custom made male-male EC5 several foot long connector cable. I know several touring couples/friends who all have female EC5 pigtails on their batteries. (Edited) Id speculate it was hooked up incorrectly.or when he was installing it, shorted out the ECU, which sits on top of the battery and can be damaged by touching a metal tool to the ECU while at the same time letting the tool contact the battery post.The Radio Control RC guys all use EC5 to charge to there are tons out there plus you can get the parts and wire (10 gauge I think?) and build you own. You just need to unplug that battery asap after a start! If you unplug the lithium jumper asap after start you can get a female EC5 pigtail w/o the extra black box. I highly recommend it, just hook it up and leave it until next time on the road. Btw I use the Deltran Battery Tender, a 5-amp smart charger that is specifically designed for AGM-batteries. You see these on the all alligator clip cables too. You can't go wrong - just make sure that the pigtail don't touch the headpipes or anything else hotter than. You need a female EC5 (connector) pigtail on your bike wired to the battery.ĪLL lithium jumpers use a male EC5 connector build into the unit.Īntigravity has a pig tail with the extra black box, so you do not back feed a charge to the battery. ![]() Telenor knows how to get them back when the customers leave, and provides the ‘Telenor Sim Lagao Deal.’ The customers receive SIM activation for the next 60 days, for 3000 On-Net minutes, 3000 MB of data. Such people can opt for daily SMS packages that are not only cost-effective but offer a decent amount of SMS as well. Telenor Daily SMS packages are typically the perfect choice for individuals who are rarely free but want to talk with their friends and family on Sundays. We will exchange Telenor SMS Packages everyday, 3 days, weekly and monthly. ![]() We recognize that these packages are difficult to locate on the company site, so we have collected numerous packages and written them on the one spot, so that we can facilitate our readers. We provide complete detail SMS packages provided by Telenor. Telenor has launched numerous SMS packages to fill the appetite of the hungry SMS generation, anticipating the ever-growing interest of today’s crowd. It will not only offer quality service but it also offers many packages that have less impact on the wallet of their customer. Telenor is now one of the top providers of cost-effective services for customers in Pakistan. All Pakistan’s Network Telenor SMS Packages include free SMS. The pattern has now changed to quick and easy text messages. It’s time to talk about long calling when people have left. Telenor has launched numerous SMS packages to satisfy the hunger of the hungry generation SMS, in anticipation of the increasing interest of the present generation. The Robot Dragon was obtainable during these Events, either in full or parts of it. ![]() This Dragon was formerly available to purchase for 8,500 from the Clockwork Dungeon Shop. Limited Breedingĭuring the time the Robot Dragon is temporarily breedable, it can be bought in exchange for Gems without prior presence on the islands. With an active VIP Status of Level 2 or higher, the breeding time is reduced to 16 hours while the hatching time is reduced to 21 hours and 20 minutes. This requires the Trainer to have reached Level 19.īreeding for this Dragon takes 20 hours while incubating and subsequently hatching the resultant egg takes 1 day, 2 hours and 40 minutes. The Robot Dragon is a Dungeon Dragon which can be obtained by entering the Bottomless Dungeon, an area which is unlocked at Level 16, and purchasing it from the Dungeon Shop in exchange for 999 Dungeon Tokens. ![]() This Dragon has the appearance of a robot, a machine that is controlled either by a computer program or has artificial intelligence. Red rectangular blocks with a silver bottom form the feet. Its tail ends in a 'C'-shape that has a red stripe running around the center the hands are shaped similarly. Running down the spine are three half arc tube lights. Above the chest are two small rectangular buttons one cyan and one orange. A pair of antennae jut out and back from the side of its head these antennae have red balls on the end and are connected to the head with movable metal bits. There is a red button on the top of its snout along with a small metal dial resting on top of the head. also with the new sigils you want epics at minimum but you will really want. ![]() I like frostbite and other dragons with wind and water. Any tips would be appreciated Agave is good because you will need to get agave to level 65 and enchant level 6 to breed orca iirc. Surrounded by a red outer ring, the bright orange eyes have a glowing yellow pupil in the center. My team right now includes Balloon dragon, Agnes dragon, and Agave dragon. The neck and trunk of the body are plated with red sheets of metal. I have added in details for breeding combinations and also their breeding times. Its head, arms, legs, and tail are covered in sheets of blue metal while its chest and underbelly are covered in sheets of light blue metal plates. Here is a list of all Dragons from Dragons Mania Legends. If you wish to disable this feature of the game, please turn off the in-app purchases in your phone or tablet's Settings.The body style of the Robot Dragon is upright. See the results of breeding combinations and find out which dragon you are breeding using the Dragon Mania Legends Breeding Calculator Select each parent by. However, you can purchase in-app items with real money. If you already love our game… Drop us a nice review!ĭragon City is free to download and free to play. Meet the electrifying Ancient Energy Dragons - The Origin of Energy event introduces a new game board and spectacular new dragons to collect - DML is. What are you waiting for? Join the game and build your city today! There are over 80 million Dragon Masters in Dragon City. A social city! Join Alliances to battle with other Dragon Masters in Dragon City, chat with them, trade Orbs in the Trading Hub, share in Alliance Gift Events, and open Alliance Chests. Unlock advanced game features like the Ancient World and build the Guardian Dragon Towers. Collect Orbs and empower your dragons: See their strength in battle grow! Summon dragons to your city from a magical world in the Tree Of Life and try their skills. Adventure through the Dragon Quests and play against other Dragon Masters in the game's PvP Arenas to collect one-of-a-kind dragons, claim Warrior's Chests, and climb the leaderboards! Decorate your dragons with cool Dragon Skins from special events. New dragons join the game every week through breeding events and special islands. Castle Events: Fight your way through dim-lit rooms, collect keys and win the ultimate dragon goal April 11-18: Imperial Egg Dragon April 25 May 2: Stone Totem Dragon. Complete the Dragon Book! Combine dragons of Fire, Nature, Pure, Legend, and many other elements, to breed unique hybrids of over 1000 awesome dragons! Strategic 3-on-3 battles that keep you engaged, whether you battle friends or the Viking Lords. ![]() Ready to master this dragon game and collect and breed tons of adorable fire-breathing dragons?īuild a Dragon City on floating islands, fill it with farms, habitats, buildings…and tons of dragons! Train them to your will, feed and evolve them into stunning monsters and prove your might to be the top Dragon Master in the world! Dozens of dragon species to discover from various elemental families, each with special powers. ![]() There are several options available that still provide the same benefit as Red Bull but with less harsh side effects. In addition, consuming excessive amounts of caffeine may cause long-term damage to vital organs such as the liver and kidneys. Too much caffeine intake can also lead to dehydration due to its diuretic properties, as well as nausea and vomiting if taken in large quantities. While these drinks can provide an energy boost, they also come with serious risks if overconsumed.Ĭaffeine is metabolized quickly by the body, which leads to increased heart rate, difficulty sleeping, headaches, and anxiety. Many people turn to energy drinks like Original Red Bull or other sugar-free options as a way to increase alertness and focus. It is important to be aware of the potential health effects that drinking too much caffeine can have. ![]() Health Effects of Excessive Caffeine IntakeĮxcessive caffeine intake can have a significant impact on an individual’s health, especially if consumed in large amounts. It’s still packed full of 80mg of caffeine but has no added sugars making it perfect for those looking to cut down on their dietary intake of sugar while still enjoying the benefits of an energy drink. On the other hand, Sugar Free Red Bull gives you all the same benefits without the calories associated with sugar. This drink provides essential vitamins, taurine, and B-group vitamins, as well as sugar for energy. Regular Red Bull is an energy drink that contains 80mg of caffeine per 8.4 fl oz can. ![]() The caffeine content in drinks is always a popular topic of discussion, and when it comes to comparing regular Red Bull vs Sugar Free Red Bull, the difference can be quite significant. Caffeine Content in Regular Red Bull vs Sugar Free Red Bull Red Bull contains 80mg of caffeine per 8.4 oz can, which is approximately 3 times more than an average cup of coffee.Ĭonsuming too much caffeine can lead to negative side effects such as restlessness and insomnia, but moderate consumption may provide beneficial performance benefits such as increased alertness or improved endurance during physical activity. Red Bull is one such energy drink that has become popular due to its energizing effects.įound in many convenience stores, gyms, and universities across the world, this beverage contains high levels of caffeine as well as other ingredients such as taurine and glucuronolactone.īut what are the effects of consuming this beverage? And how much caffeine does it contain?
She once believed their connection was a “most rare and special thing” in the vicinity of her former husband, and as solitary and deep in thought, she concludes, “our love was doomed from the start.” As she reminisces about a terrible road trip to Vancouver in 1938 and when they tied the knot (“Eventually, August and I decided to wed. June views August as “the force who most shaped path.” His character and their ill-fated marriage intrude into her thoughts as well. June discloses her intense love for Leora as well as her anguish when Leora lost a limb to a cancer that eventually recurred. She touches on her history (a village near Vienna, parents killed in a concentration camp), career, eventual residence in England, and “nomadic life.” Often, though, her reflections settle on her daughter. June’s musings in alternating chapters are a marked contrast. ![]() With that said, when news reaches him about his child’s illness, August’s driven to work on his “cobalt machine” to help cure her. Generally, lab projects, acclaim, rivalries, and wealth quicken his step. A touch megalomaniacal, he once told June, “I want to be remembered as the man who saved the world.” The chapters narrated by Snow do acknowledge June and Leora, but word-for-word the emphasis isn’t marital dynamics, nor matters of the heart. As Hungary swooped right, he changed his name and official religious status, fled to Germany, and became a student of Einstein.Īugust recalls career opportunities in London and then Chicago and illustrates his passions - abundant cheap energy, the fission reactor. For him, “the beginning” was the Great War, when he resided in Pest (then “distinct from bourgeois Buda”). Fiction enabled the author to explore ways Weiss - who “seemed like a victim in her marriage” - “might have found her power.”Īugust is nevertheless the novel’s first and final perspective. Lye notes that very little of the voice and life of Szilard’s wife, Trude Weiss, were preserved. Inspired by Leo Szilard, a Hungarian-born physicist known for his integral work on (and patents for) nuclear fission, “Let It Destroy You” examines idealism and ambition, intent and outcome, and, with particular acuity, marriage and the complex, unpredictable ways its faltering can play out. Lucrative patents have altered public impressions of Snow’s scientific enterprises with good reason, June is burdened by thoughts of her own culpability. Though a physician, June assisted her husband with paperwork, such as patent applications. At the same time and date, June’s thoughts skip from Leora, their daughter, to the circumstances that led to August’s trial. ![]() And: “Perception is everything.” He resigns himself to recollection: “I may as well start at the beginning.”Ī dextrous hand at mood and pace, Toronto writer Lye (debut novel “The Honey Farm” and memoir “ Natural Killer”) begins the next chapter with “I am here only as a witness.” The speaker is June, estranged wife of August Snow, the prisoner. Whatever the verdict, his life will be lessened. His trial, at the Hague, is set to commence the following day. It is why they are calling me a war criminal.” “But it is late summer, and what falls is not snow, but ash,” the narrator thinks. As night encroaches, he peers at distant rolling hills and discerns lightly falling snow. on Auga scientist waits in a holding cell. Harriet Alida Lye opens “Let It Destroy You,” her sophomore novel, with a confidently dramatic flourish. An intermediate-purity virus preparation is achieved by adding a high speed ultracentrifugation step. For example, cellular proteases could degrade virus, while cytokines could alter cell signalling and affect susceptibility to viruses. While this approach is quick and permits simultaneous high-throughput comparisons of a large sundry of viruses, it suffers from high contamination with non-virus factors that may affect experimental outcomes ( Table 1). To generate lysates, cells are commonly lysed by freeze-thaw, debris is removed by low speed centrifugation, and supernatants are then used for experimentation. The most rapid approach involves using crude cell lysates or cell culture media from virus-infected cells. Most laboratories use one of three methods to prepare virus for experimentation. ![]() The in-slurry purification approach offered substantially increased virus purity over crude cell lysates, media, or high-spin preparations and would be especially useful for high-throughput virus screening applications where density gradient ultracentrifugation is not feasible. Capto Core 700 resin was then effectively adapted to a rapid in-slurry pull-out approach for high-throughput purification of reovirus and adenovirus. Core 700 chromatography produced virion purity and infectivity indistinguishable from CsCl density gradient ultracentrifugation as determined by electron microscopy, gel electrophoresis analysis and plaque titration. ![]() To overcome this shortcoming, we evaluated a commercially available resin (Capto Core 700) that captures molecules smaller than 700 kDa. Our research pace was limited by the lack of high-throughput virus purification methods that efficiently remove confounding cellular contaminants such as cytokines and proteases. Our laboratory explores mutations in oncolytic reovirus that could improve oncolytic activity, and makes routine use of numerous virus variants, genome reassortants, and reverse engineered mutants. ![]() Existing methods to purify viruses such as gradient ultracentrifugation or chromatography have limitations, for example demand for technical expertise or specialized equipment, high time consumption, and restricted capacity. Viruses are extensively studied as pathogens and exploited as molecular tools and therapeutic agents. |
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